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Operational Manual for Bentchtop Autoclaver

A collection of operational manuals for essential instrument in microbiology/microbial genomics laboratories.

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Table of Contents

Key Specs

  • A potable steam pressure sterilizer made of stainless steel.

  • Total volume 18L, 2kw, max. 28 cm ⌀, 23.5 cm – height.

  • 121-126 °C, 0.11-0.142 MPa.

  • Safety first, always ask before use.

Instrument Overview
autoclaver
Manual
1. Make sure the water discharge valve is tightly closed off.
valve
2. Rotate open the lid of the autoclaver. Add ~1.6 L deminalized pure water into the chamber.
water
3. Visually check the water level to make sure the small floating cell used as the water level indicator hits the top, but not being completely submerged.
check
4. Put the steel load bucket in and rotate close the lid. Make sure the pressure sensor is placed at the right location.
sensor
5. Set a right pressure and an appropriate time by turning the knobs. Commonly set as 121 °C for 15-30 mins for most applications in microbiology.
knobs
6. Make sure the lid is positioned correctly and then power on. Once finished, an alarm will ring. Power off and wait till the autoclaver cools down to room temperature (ca. 30-60 mins) before opening. 
DO NOT OPEN IT WHEN IT IS STILL HOT. STEAM MAY CAUSE INJURY!
autoclaver-s

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