Emergent Sequencing

Genome sequencing often takes too long from DNA prep to final insights into the generated microbial genomics data. Emergent sequencing service addresses situations where you wish to receive the results within a week and are willing to afford additional costs.

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Table of Contents

Key Specs
  • Only address urgent sequencing needs outside medical applications.

  • Using the Nanopore sequencer in our lab or the core sequencing facilities at the University of Copenhagen.

  • The service fee is calculated as any consumables and sequencer running costs at transparent, original prices + 600 kr./hour (€ 79) fixed rate for labour cost + a one-time setup charge of 999 kr. (€ 139).

  • Turnaround time: max. one week, details upon inquiry. This service is unavailable during holidays in Denmark, UK, and China.

Sample Requirements

For DNA samples submitted, Customer is required to ensure the Quality Control (QC) data at the time of sample submission meets the minimum requirements concerning the total amount, concentration, and quality of the DNA submitted. For microbial culture or direct environmental samples submitted, Customer is obligated to ensure that the sample submitted is non-pathogenic to humans and does not contain any substance that may pose a health risk to the personnel working with the samples in our laboratory.

DNA sample
  • Total quantity ≥ 1.0 μg (short-insert library) or ≥ 5 μg (PCR-free library);

  • Concentration ≥ 20 ng/µL;

  • Purity: A260/280=1.8-2.0.



(1)  The final concentration of each sample will be measured using QUBIT or an equivalent fluorometer after the samples are received. If the samples do not pass QC, We will notify the Customer, and Customer shall either notify us to cancel the failed samples or re-send the samples concerned within ten workdays. Customer can request that the “QC-failed samples” be sequenced, in which case we will prepare the library and sequence the “QC-failed samples” without bearing any responsibility for the data quality. Customer should notify whether plasmid, chondriosome, chloroplast, and other non-bacterial-origin DNA exist in the samples submitted. Customer should notice that, If the DNA originates from a mixture of multiple microbial species, the quality of final assembly and genome annotation will be compromised significantly, and we will not attempt to separate all assemblies into individual species by any means.

(2)  An aliquot of DNA sample may be stored temporarily in case of a failed library preparation. All DNA samples will be discarded within 3 months after the project is completed.

Microbial pure culture sample

Agar plates: 

colonies should be easily visible and cover > 20% of the surface area on a standard-size petri dish (⌀ 90mm); the agar should not dry out and maintain a good texture for easy collection of cells with a standard sterile loop used in microbiology laboratories.


Bacterial pellets should be placed in a 1.5 mL or 2 mL centrifuge tube. The size of the pellet shall not be less than that generated by centrifuging 2 mL of E. coli cultures grown in LB overnight at 37 °C, typically covering a round area of ⌀3-5 mm.


Customer should pay close attention to contamination on agar plates or in bacterial pellets. Customer must ensure each sample submitted is a single pure culture without being mixed with other species. In any case, we will continue the workflow and sequence the genomic DNA extracted from the samples submitted. We will not accept the complaint on the culture’s purity issue filed by Customer based on the genome sequence data generated in this project.

Environmental sample

The direct submission of soil, lake water, filters, swabs, plant or animal materials, any items or objects colonized by microbes, and other types of environmental samples that contain microbial inhabitants for genomic investigation at our laboratory is subject to individual agreement by email or in other written form between Customer and us concerning the sample type, quantity, and quality before the commence of this agreement.


A project will only commence when we reach an agreement and receive the last batch of qualified samples. The deliverables are the same as in standard sequencing service packages.

Sample deliverables

For details, the Customer is referred to the corresponding sequencing service packages. Here we use amplicon sequencing as an example.

  • DNA QC report
Pricing & Turnaround Time

Sequencing costs vary significantly among different projects, please contact us for more details. Typically, an emergency project costs 3~5 times more than a standard project. This is how we calculate the cost of a project:

[consumables and sequencer running costs] + 600 kr./hour (€ 79) fixed rate for labour cost + a one-time setup charge of 999 kr. (€ 139)

For the consumables and sequencer running costs, we use the online shop price of corresponding suppliers. Please check the following documents for current rates.

The project turnaround time is max. one week. Please be aware that we are an international team; thus, this service is unavailable during national holidays in Denmark, UK, and China.


Prices are retrieved from the corresponding websites without guarantee accuracy and may change with prior notice.

Add-on Services

The Pro Bioinformatics Package (1,699 kr.; € 229) include all Pro services listed on the workflow. The Premium Bioinformatics Package (9,199 kr.; € 1,299) is highly customer-tailored with the highest level of services provided to address any significant challenges facing customers in their applications; details upon inquiry.

Payment Term

All payments set forth on the invoice are due within 30 days from the date of the invoice. We may impose a service charge on past due amounts between one and one-half percent (1.5%) and three percent (3%) per month, not to exceed the maximum amount permitted by law. Upon the Customer’s request, we will provide a statement within ten days.

The service agreement should be governed by and construed in accordance with the law in Denmark.

Service Contract

A draft agreement with amplicon sequencing as an example

Q: Can I bring samples to your lab to expedite the process?

I would highly recommend that you do that. This will help to shorten the time needed to complete the project. We are also happy to work together in the lab with our customers to reach the best outcome in the shortest possible time. 

Q: How can I have a good guess of the cost before reaching out to you for a quote?

We adopt a transparent pricing policy for all customers, whether you are a big pharma, a public institution, or a small startup. This also applies to emergent sequencing services. Please refer to the current rates shown above for consumables and sequencer running costs. The Customers are encouraged to do their own calculations. We will refund the difference overcharged if there are any deviations between your calculations and ours. In essence, we only charge labour costs and a one-off setup fee.

Q: How can you ensure a fast return of the results from bioinformatics analysis?

This is guaranteed, as we state in our service targets, because we have our own bioinformatics servers in-house with automated pipelines, which are constantly monitored remotely by a small but highly capable and efficient data scientist team. The only thing is that we need to adjust the priority of existing projects to serve emergency projects better.

See more at mBioWorks FAQ.

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