{"id":8273,"date":"2024-04-22T23:31:34","date_gmt":"2024-04-22T22:31:34","guid":{"rendered":"https:\/\/mbioworks.com\/?p=8273"},"modified":"2025-04-09T11:36:07","modified_gmt":"2025-04-09T10:36:07","slug":"microbial-community-profiling-2-2-2","status":"publish","type":"post","link":"https:\/\/mbioworks.com\/?p=8273","title":{"rendered":"DNA Extraction From Microbiomes"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"8273\" class=\"elementor elementor-8273\">\n\t\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-27db53e elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"27db53e\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-5e8675f\" data-id=\"5e8675f\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-5364571 elementor-widget elementor-widget-text-editor\" data-id=\"5364571\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.11.5 - 14-03-2023 *\/\n.elementor-widget-text-editor.elementor-drop-cap-view-stacked .elementor-drop-cap{background-color:#818a91;color:#fff}.elementor-widget-text-editor.elementor-drop-cap-view-framed .elementor-drop-cap{color:#818a91;border:3px solid;background-color:transparent}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap{margin-top:8px}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap-letter{width:1em;height:1em}.elementor-widget-text-editor .elementor-drop-cap{float:left;text-align:center;line-height:1;font-size:50px}.elementor-widget-text-editor .elementor-drop-cap-letter{display:inline-block}<\/style>\t\t\t\t<h5><b>Key Specs<\/b><\/h5>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5657fce elementor-widget elementor-widget-text-editor\" data-id=\"5657fce\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<ul><li><p>A complete workflow from cell chemical\/physical disruption to enzyme or beads-based DNA purification.<\/p><\/li><li><p><span style=\"color: #003366\"><span style=\"white-space: pre-wrap\">Working on various microbiome samples, including but not limited to soils, lake waters, air, blood, poops, saliva, plastic bags, skin, plant or animal tissues, private homes, public spaces, etc.<\/span><\/span><\/p><\/li><li><p><span style=\"color: #003366\"><span style=\"white-space: pre-wrap\">Carried out in mBioWorks&#8217; Copenhagen Laboratory, either manually or with an automatic nucleotide acid extractor. Visits are welcome.<\/span><\/span><\/p><\/li><\/ul>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-14bc6b9 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"14bc6b9\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-d34964f\" data-id=\"d34964f\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-de0ffe3 elementor-widget elementor-widget-text-editor\" data-id=\"de0ffe3\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<h5><b>Microbiome<\/b><\/h5>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5d37598 elementor-widget elementor-widget-image\" data-id=\"5d37598\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.11.5 - 14-03-2023 *\/\n.elementor-widget-image{text-align:center}.elementor-widget-image a{display:inline-block}.elementor-widget-image a img[src$=\".svg\"]{width:48px}.elementor-widget-image img{vertical-align:middle;display:inline-block}<\/style>\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/microbiome-mbioworks-scaled.jpg\" title=\"\" alt=\"\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-37c5276 elementor-widget elementor-widget-image\" data-id=\"37c5276\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"2047\" height=\"1137\" src=\"https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/microbiome-mbioworks2.jpg\" class=\"attachment-full size-full wp-image-8280\" alt=\"\" srcset=\"https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/microbiome-mbioworks2.jpg 2047w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/microbiome-mbioworks2-300x167.jpg 300w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/microbiome-mbioworks2-1024x569.jpg 1024w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/microbiome-mbioworks2-768x427.jpg 768w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/microbiome-mbioworks2-1536x853.jpg 1536w\" sizes=\"auto, (max-width: 2047px) 100vw, 2047px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-43bc81d elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"43bc81d\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-495bd80\" data-id=\"495bd80\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-8a7c310 elementor-widget elementor-widget-text-editor\" data-id=\"8a7c310\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<h5><b>Sample Requirements<\/b><\/h5>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-880f610 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"880f610\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-bd8160c\" data-id=\"bd8160c\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-8765ead elementor-widget elementor-widget-text-editor\" data-id=\"8765ead\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>The direct submission of soil, lake water, filters, swabs, plant or animal materials, any items or objects colonized by microbes, and other types of environmental samples that contain microbial inhabitants is subject to prior agreement between Customer and us concerning the sample type, quantity, and quality. Microbiome samples can vary a lot in physical-chemical properties and packaging and shipping requirements. Please contact us for more details.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-eee49a9 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"eee49a9\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-bbafaef\" data-id=\"bbafaef\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-10213c8 elementor-widget elementor-widget-text-editor\" data-id=\"10213c8\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<h5><b>Methods<\/b><\/h5>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-4172c8f elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"4172c8f\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-fa4fb98\" data-id=\"fa4fb98\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-3d9f690 elementor-widget elementor-widget-text-editor\" data-id=\"3d9f690\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>There are several commonly used methods for DNA extraction from microbial or environmental samples. These methods are designed to be user-friendly and efficient, generating high-quality DNA for downstream applications.\u00a0<\/p><p>\u00a0<\/p><h6><strong style=\"color: inherit;font-family: inherit;font-size: 1rem;background-color: var( --e-global-color-f898f31 )\">Phenol-chloroform extraction:<\/strong><\/h6><p>This traditional method relies on the differential solubility of DNA in aqueous and organic phases. The bacterial culture is first lysed using enzymes, heat, or detergents, releasing the DNA. Then, a mixture of phenol, chloroform, and isoamyl alcohol is added, followed by centrifugation to separate the aqueous (upper) and organic (lower) phases. DNA remains in the aqueous phase, while proteins and other cellular debris move into the organic phase. The DNA is then precipitated using ethanol or isopropanol, washed, and dissolved in an appropriate buffer.<\/p><p>Pros:<\/p><ul><li>Well-established method<\/li><li>Yields high-quality DNA<\/li><\/ul><p>Cons:<\/p><ul><li>Labor-intensive<\/li><li>Involves hazardous chemicals<\/li><\/ul><h6>\u00a0<\/h6><h6><strong>Silica membrane-based column extraction:<\/strong><\/h6><p>In this method, bacterial cells are lysed using a combination of enzymatic and chemical treatments. The resulting lysate is then mixed with a chaotropic salt, which helps the DNA bind to a silica membrane in a spin column. The silica membrane selectively binds DNA when the lysate is passed through the column. After washing away contaminants, the DNA is eluted using a low-salt buffer or water.<\/p><p>The ability of DNA to bind to silica surfaces is due to the negatively charged phosphate backbone of the DNA molecule, which forms ionic interactions with the positively charged silica surface. The presence of chaotropic salts helps facilitate this binding by disrupting the hydration shell of both DNA and silica, enhancing their interaction.<\/p><p>Pros:<\/p><ul><li>Simple and fast<\/li><li>Yields high-quality DNA<\/li><li>Doesn&#8217;t require hazardous chemicals<\/li><\/ul><p>Cons:<\/p><ul><li>Can be less efficient for certain samples (e.g., high GC content)<\/li><\/ul><h6><strong style=\"color: inherit;font-family: inherit;font-size: 1rem;background-color: var( --e-global-color-f898f31 )\">\u00a0<\/strong><\/h6><h6><strong style=\"color: inherit;font-family: inherit;font-size: 1rem;background-color: var( --e-global-color-f898f31 )\">Magnetic bead-based extraction:<\/strong><\/h6><p>This method also relies on chaotropic salts for lysing bacterial cells and liberating DNA. The DNA then binds to magnetic beads in the presence of a chaotropic salt. The bead-bound DNA is separated from contaminants using a magnetic separator. After washing, the purified DNA is eluted in a low-salt buffer or water.<\/p><p>The principle of DNA binding to magnetic beads is similar to that of silica membrane-based extraction. Magnetic beads are coated with functional groups that can interact with the negatively charged phosphate backbone of DNA molecules. The presence of chaotropic salts enhances the binding by disrupting the hydration shell of DNA and the coated magnetic beads.<\/p><p>Pros:<\/p><ul><li>Highly scalable (suitable for high-throughput applications)<\/li><li>Yields high-quality DNA<\/li><li>Doesn&#8217;t require hazardous chemicals<\/li><\/ul><p>Cons:<\/p><ul><li>Requires specialized equipment (magnetic separator)<\/li><\/ul><p>\u00a0<\/p><p>In summary, the phenol-chloroform method is a traditional approach for DNA extraction but involves hazardous chemicals and is labour-intensive. Silica membrane-based column extraction and magnetic bead-based extraction are more user-friendly and safer alternatives, with both methods providing high-quality DNA. The choice between these methods depends on factors like sample type, desired throughput, and available equipment. At mBioWorks, we use either silica membrane or magnetic bead-based method depending on a specific project.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-2ed4483 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"2ed4483\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-e133973\" data-id=\"e133973\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6981154 elementor-widget elementor-widget-text-editor\" data-id=\"6981154\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<h5><b>Protocols<\/b><\/h5>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-a24a4b8 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"a24a4b8\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-3fa9e58\" data-id=\"3fa9e58\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-478bc1b elementor-widget elementor-widget-text-editor\" data-id=\"478bc1b\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<h6><a href=\"https:\/\/mbioworks.com\/?attachment_id=8289\">QIAGEN DNeasy PowerSoil DNA Isolation Kit<\/a><\/h6>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e1a0458 gallery-spacing-custom elementor-widget elementor-widget-image-gallery\" data-id=\"e1a0458\" data-element_type=\"widget\" data-widget_type=\"image-gallery.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.11.5 - 14-03-2023 *\/\n.elementor-image-gallery .gallery-item{display:inline-block;text-align:center;vertical-align:top;width:100%;max-width:100%;margin:0 auto}.elementor-image-gallery .gallery-item img{margin:0 auto}.elementor-image-gallery .gallery-item .gallery-caption{margin:0}.elementor-image-gallery figure img{display:block}.elementor-image-gallery figure figcaption{width:100%}.gallery-spacing-custom .elementor-image-gallery .gallery-icon{padding:0}@media (min-width:768px){.elementor-image-gallery .gallery-columns-2 .gallery-item{max-width:50%}.elementor-image-gallery .gallery-columns-3 .gallery-item{max-width:33.33%}.elementor-image-gallery .gallery-columns-4 .gallery-item{max-width:25%}.elementor-image-gallery .gallery-columns-5 .gallery-item{max-width:20%}.elementor-image-gallery .gallery-columns-6 .gallery-item{max-width:16.666%}.elementor-image-gallery .gallery-columns-7 .gallery-item{max-width:14.28%}.elementor-image-gallery .gallery-columns-8 .gallery-item{max-width:12.5%}.elementor-image-gallery .gallery-columns-9 .gallery-item{max-width:11.11%}.elementor-image-gallery .gallery-columns-10 .gallery-item{max-width:10%}}@media (min-width:480px) and (max-width:767px){.elementor-image-gallery .gallery.gallery-columns-2 .gallery-item,.elementor-image-gallery .gallery.gallery-columns-3 .gallery-item,.elementor-image-gallery .gallery.gallery-columns-4 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.gallery-item,.elementor-image-gallery .gallery.gallery-columns-10 .gallery-item{max-width:100%}}<\/style>\t\t<div class=\"elementor-image-gallery\">\n\t\t\t<div id='gallery-1' class='gallery galleryid-8273 gallery-columns-4 gallery-size-full'><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-slideshow=\"e1a0458\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6ODI4NywidXJsIjoiaHR0cHM6XC9cL21iaW93b3Jrcy5jb21cL3dwLWNvbnRlbnRcL3VwbG9hZHNcLzIwMjNcLzA0XC9IQi0yNDk0LTAwM18xMTE3NTY5X1BjYXJkX0ROWV9Qb3dlclNvaWxfUHJvXzA1MTlfV1dfUGFnZV8xLmpwZyIsInNsaWRlc2hvdyI6ImUxYTA0NTgifQ%3D%3D\" href='https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/HB-2494-003_1117569_Pcard_DNY_PowerSoil_Pro_0519_WW_Page_1.jpg'><img loading=\"lazy\" decoding=\"async\" width=\"1749\" height=\"2481\" src=\"https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/HB-2494-003_1117569_Pcard_DNY_PowerSoil_Pro_0519_WW_Page_1.jpg\" class=\"attachment-full size-full\" alt=\"\" srcset=\"https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/HB-2494-003_1117569_Pcard_DNY_PowerSoil_Pro_0519_WW_Page_1.jpg 1749w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/HB-2494-003_1117569_Pcard_DNY_PowerSoil_Pro_0519_WW_Page_1-211x300.jpg 211w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/HB-2494-003_1117569_Pcard_DNY_PowerSoil_Pro_0519_WW_Page_1-722x1024.jpg 722w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/HB-2494-003_1117569_Pcard_DNY_PowerSoil_Pro_0519_WW_Page_1-768x1089.jpg 768w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/HB-2494-003_1117569_Pcard_DNY_PowerSoil_Pro_0519_WW_Page_1-1083x1536.jpg 1083w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/HB-2494-003_1117569_Pcard_DNY_PowerSoil_Pro_0519_WW_Page_1-1444x2048.jpg 1444w\" sizes=\"auto, (max-width: 1749px) 100vw, 1749px\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-slideshow=\"e1a0458\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6ODI4OCwidXJsIjoiaHR0cHM6XC9cL21iaW93b3Jrcy5jb21cL3dwLWNvbnRlbnRcL3VwbG9hZHNcLzIwMjNcLzA0XC9IQi0yNDk0LTAwM18xMTE3NTY5X1BjYXJkX0ROWV9Qb3dlclNvaWxfUHJvXzA1MTlfV1dfUGFnZV8yLmpwZyIsInNsaWRlc2hvdyI6ImUxYTA0NTgifQ%3D%3D\" href='https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/HB-2494-003_1117569_Pcard_DNY_PowerSoil_Pro_0519_WW_Page_2.jpg'><img loading=\"lazy\" decoding=\"async\" width=\"1749\" height=\"2481\" src=\"https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/HB-2494-003_1117569_Pcard_DNY_PowerSoil_Pro_0519_WW_Page_2.jpg\" class=\"attachment-full size-full\" alt=\"\" srcset=\"https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/HB-2494-003_1117569_Pcard_DNY_PowerSoil_Pro_0519_WW_Page_2.jpg 1749w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/HB-2494-003_1117569_Pcard_DNY_PowerSoil_Pro_0519_WW_Page_2-211x300.jpg 211w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/HB-2494-003_1117569_Pcard_DNY_PowerSoil_Pro_0519_WW_Page_2-722x1024.jpg 722w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/HB-2494-003_1117569_Pcard_DNY_PowerSoil_Pro_0519_WW_Page_2-768x1089.jpg 768w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/HB-2494-003_1117569_Pcard_DNY_PowerSoil_Pro_0519_WW_Page_2-1083x1536.jpg 1083w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/HB-2494-003_1117569_Pcard_DNY_PowerSoil_Pro_0519_WW_Page_2-1444x2048.jpg 1444w\" sizes=\"auto, (max-width: 1749px) 100vw, 1749px\" \/><\/a>\n\t\t\t<\/div><\/figure>\n\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-0ff0dc0 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"0ff0dc0\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-d8a6ca0\" data-id=\"d8a6ca0\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-a1795ce elementor-widget elementor-widget-text-editor\" data-id=\"a1795ce\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<h6><a href=\"https:\/\/mbioworks.com\/?attachment_id=8290\">VAZYME FastPure Microbiome DNA Isolation Kit<\/a><\/h6>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d25f5e1 gallery-spacing-custom elementor-widget elementor-widget-image-gallery\" data-id=\"d25f5e1\" data-element_type=\"widget\" data-widget_type=\"image-gallery.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-image-gallery\">\n\t\t\t<div id='gallery-2' class='gallery galleryid-8273 gallery-columns-4 gallery-size-full'><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-slideshow=\"d25f5e1\" 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https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/FastPure-Microbiome-DNA-Isolation-Kit-Manual_Page_2-724x1024.jpg 724w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/FastPure-Microbiome-DNA-Isolation-Kit-Manual_Page_2-768x1086.jpg 768w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/FastPure-Microbiome-DNA-Isolation-Kit-Manual_Page_2-1086x1536.jpg 1086w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/FastPure-Microbiome-DNA-Isolation-Kit-Manual_Page_2-1449x2048.jpg 1449w\" sizes=\"auto, (max-width: 1811px) 100vw, 1811px\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-slideshow=\"d25f5e1\" 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https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/FastPure-Microbiome-DNA-Isolation-Kit-Manual_Page_4-724x1024.jpg 724w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/FastPure-Microbiome-DNA-Isolation-Kit-Manual_Page_4-768x1086.jpg 768w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/FastPure-Microbiome-DNA-Isolation-Kit-Manual_Page_4-1086x1536.jpg 1086w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/FastPure-Microbiome-DNA-Isolation-Kit-Manual_Page_4-1449x2048.jpg 1449w\" sizes=\"auto, (max-width: 1811px) 100vw, 1811px\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-slideshow=\"d25f5e1\" 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https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/FastPure-Microbiome-DNA-Isolation-Kit-Manual_Page_5-724x1024.jpg 724w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/FastPure-Microbiome-DNA-Isolation-Kit-Manual_Page_5-768x1086.jpg 768w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/FastPure-Microbiome-DNA-Isolation-Kit-Manual_Page_5-1086x1536.jpg 1086w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/FastPure-Microbiome-DNA-Isolation-Kit-Manual_Page_5-1449x2048.jpg 1449w\" sizes=\"auto, (max-width: 1811px) 100vw, 1811px\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-slideshow=\"d25f5e1\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6ODI5NiwidXJsIjoiaHR0cHM6XC9cL21iaW93b3Jrcy5jb21cL3dwLWNvbnRlbnRcL3VwbG9hZHNcLzIwMjNcLzA0XC9GYXN0UHVyZS1NaWNyb2Jpb21lLUROQS1Jc29sYXRpb24tS2l0LU1hbnVhbF9QYWdlXzYtc2NhbGVkLmpwZyIsInNsaWRlc2hvdyI6ImQyNWY1ZTEifQ%3D%3D\" href='https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/FastPure-Microbiome-DNA-Isolation-Kit-Manual_Page_6-scaled.jpg'><img loading=\"lazy\" decoding=\"async\" width=\"1811\" height=\"2560\" src=\"https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/FastPure-Microbiome-DNA-Isolation-Kit-Manual_Page_6-scaled.jpg\" class=\"attachment-full size-full\" alt=\"\" srcset=\"https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/FastPure-Microbiome-DNA-Isolation-Kit-Manual_Page_6-scaled.jpg 1811w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/FastPure-Microbiome-DNA-Isolation-Kit-Manual_Page_6-212x300.jpg 212w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/FastPure-Microbiome-DNA-Isolation-Kit-Manual_Page_6-724x1024.jpg 724w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/FastPure-Microbiome-DNA-Isolation-Kit-Manual_Page_6-768x1086.jpg 768w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/FastPure-Microbiome-DNA-Isolation-Kit-Manual_Page_6-1086x1536.jpg 1086w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/FastPure-Microbiome-DNA-Isolation-Kit-Manual_Page_6-1449x2048.jpg 1449w\" sizes=\"auto, (max-width: 1811px) 100vw, 1811px\" \/><\/a>\n\t\t\t<\/div><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-slideshow=\"d25f5e1\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6ODI5NywidXJsIjoiaHR0cHM6XC9cL21iaW93b3Jrcy5jb21cL3dwLWNvbnRlbnRcL3VwbG9hZHNcLzIwMjNcLzA0XC9GYXN0UHVyZS1NaWNyb2Jpb21lLUROQS1Jc29sYXRpb24tS2l0LU1hbnVhbF9QYWdlXzctc2NhbGVkLmpwZyIsInNsaWRlc2hvdyI6ImQyNWY1ZTEifQ%3D%3D\" href='https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/FastPure-Microbiome-DNA-Isolation-Kit-Manual_Page_7-scaled.jpg'><img loading=\"lazy\" decoding=\"async\" width=\"1811\" height=\"2560\" src=\"https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/FastPure-Microbiome-DNA-Isolation-Kit-Manual_Page_7-scaled.jpg\" class=\"attachment-full size-full\" alt=\"\" srcset=\"https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/FastPure-Microbiome-DNA-Isolation-Kit-Manual_Page_7-scaled.jpg 1811w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/FastPure-Microbiome-DNA-Isolation-Kit-Manual_Page_7-212x300.jpg 212w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/FastPure-Microbiome-DNA-Isolation-Kit-Manual_Page_7-724x1024.jpg 724w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/FastPure-Microbiome-DNA-Isolation-Kit-Manual_Page_7-768x1086.jpg 768w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/FastPure-Microbiome-DNA-Isolation-Kit-Manual_Page_7-1086x1536.jpg 1086w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/FastPure-Microbiome-DNA-Isolation-Kit-Manual_Page_7-1449x2048.jpg 1449w\" sizes=\"auto, (max-width: 1811px) 100vw, 1811px\" \/><\/a>\n\t\t\t<\/div><\/figure>\n\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-400ab9d elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"400ab9d\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-bd7bdb8\" data-id=\"bd7bdb8\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6fde152 elementor-widget elementor-widget-text-editor\" data-id=\"6fde152\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<h5><b>Key Equipment<\/b><\/h5>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-01e3360 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"01e3360\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-26c4eb1\" data-id=\"26c4eb1\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-35e6b02 gallery-spacing-custom elementor-widget elementor-widget-image-gallery\" data-id=\"35e6b02\" data-element_type=\"widget\" data-widget_type=\"image-gallery.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-image-gallery\">\n\t\t\t<div id='gallery-3' class='gallery galleryid-8273 gallery-columns-4 gallery-size-full'><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-slideshow=\"35e6b02\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6ODI0MywidXJsIjoiaHR0cHM6XC9cL21iaW93b3Jrcy5jb21cL3dwLWNvbnRlbnRcL3VwbG9hZHNcLzIwMjNcLzA0XC8yMi5qcGciLCJzbGlkZXNob3ciOiIzNWU2YjAyIn0%3D\" href='https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/22.jpg'><img loading=\"lazy\" decoding=\"async\" width=\"1200\" height=\"1309\" src=\"https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/22.jpg\" class=\"attachment-full size-full\" alt=\"\" aria-describedby=\"gallery-3-8243\" srcset=\"https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/22.jpg 1200w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/22-275x300.jpg 275w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/22-939x1024.jpg 939w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/22-768x838.jpg 768w\" sizes=\"auto, (max-width: 1200px) 100vw, 1200px\" \/><\/a>\n\t\t\t<\/div>\n\t\t\t\t<figcaption class='wp-caption-text gallery-caption' id='gallery-3-8243'>\n\t\t\t\tNano-Spectrophotometer For DNA QC\n\t\t\t\t<\/figcaption><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-slideshow=\"35e6b02\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6ODI0NCwidXJsIjoiaHR0cHM6XC9cL21iaW93b3Jrcy5jb21cL3dwLWNvbnRlbnRcL3VwbG9hZHNcLzIwMjNcLzA0XC8xMTEuanBnIiwic2xpZGVzaG93IjoiMzVlNmIwMiJ9\" href='https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/111.jpg'><img loading=\"lazy\" decoding=\"async\" width=\"1200\" height=\"1309\" src=\"https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/111.jpg\" class=\"attachment-full size-full\" alt=\"\" aria-describedby=\"gallery-3-8244\" srcset=\"https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/111.jpg 1200w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/111-275x300.jpg 275w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/111-939x1024.jpg 939w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/111-768x838.jpg 768w\" sizes=\"auto, (max-width: 1200px) 100vw, 1200px\" \/><\/a>\n\t\t\t<\/div>\n\t\t\t\t<figcaption class='wp-caption-text gallery-caption' id='gallery-3-8244'>\n\t\t\t\tAutomatic Nucleic Acid Extractor For 32 Samples\n\t\t\t\t<\/figcaption><\/figure>\n\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>High-quality DNA extraction is crucial for any microbiome sequencing project. At mBioWorks, we use reliable and cost-effective commercial microbiome DNA extraction kits to isolate pure DNA from all types of microbiome samples.<\/p>\n","protected":false},"author":2,"featured_media":8338,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[60],"tags":[34,27,25,26],"class_list":["post-8273","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-wetlab","tag-dna","tag-sample","tag-service","tag-workflow"],"_links":{"self":[{"href":"https:\/\/mbioworks.com\/index.php?rest_route=\/wp\/v2\/posts\/8273","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mbioworks.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/mbioworks.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/mbioworks.com\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/mbioworks.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=8273"}],"version-history":[{"count":13,"href":"https:\/\/mbioworks.com\/index.php?rest_route=\/wp\/v2\/posts\/8273\/revisions"}],"predecessor-version":[{"id":8339,"href":"https:\/\/mbioworks.com\/index.php?rest_route=\/wp\/v2\/posts\/8273\/revisions\/8339"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/mbioworks.com\/index.php?rest_route=\/wp\/v2\/media\/8338"}],"wp:attachment":[{"href":"https:\/\/mbioworks.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=8273"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/mbioworks.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=8273"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/mbioworks.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=8273"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}