{"id":8092,"date":"2024-04-21T10:17:04","date_gmt":"2024-04-21T09:17:04","guid":{"rendered":"https:\/\/mbioworks.com\/?p=8092"},"modified":"2025-04-09T11:36:18","modified_gmt":"2025-04-09T10:36:18","slug":"wetlab-protocols","status":"publish","type":"post","link":"https:\/\/mbioworks.com\/?p=8092","title":{"rendered":"WetLab Protocols"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"8092\" class=\"elementor elementor-8092\">\n\t\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-d36f5a2 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"d36f5a2\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-619c63c\" data-id=\"619c63c\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-7991b5f elementor-widget elementor-widget-heading\" data-id=\"7991b5f\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.11.5 - 14-03-2023 *\/\n.elementor-heading-title{padding:0;margin:0;line-height:1}.elementor-widget-heading .elementor-heading-title[class*=elementor-size-]>a{color:inherit;font-size:inherit;line-height:inherit}.elementor-widget-heading .elementor-heading-title.elementor-size-small{font-size:15px}.elementor-widget-heading .elementor-heading-title.elementor-size-medium{font-size:19px}.elementor-widget-heading .elementor-heading-title.elementor-size-large{font-size:29px}.elementor-widget-heading .elementor-heading-title.elementor-size-xl{font-size:39px}.elementor-widget-heading .elementor-heading-title.elementor-size-xxl{font-size:59px}<\/style><h6 class=\"elementor-heading-title elementor-size-default\"><b>A Collection of Protocols Used in the mBioWorks Wet Laboratory<\/b><\/h6>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-fd0b774 elementor-grid-3 elementor-grid-tablet-2 elementor-grid-mobile-1 elementor-posts--thumbnail-top elementor-widget elementor-widget-posts\" data-id=\"fd0b774\" data-element_type=\"widget\" data-settings=\"{&quot;pagination_type&quot;:&quot;numbers_and_prev_next&quot;,&quot;classic_columns&quot;:&quot;3&quot;,&quot;classic_columns_tablet&quot;:&quot;2&quot;,&quot;classic_columns_mobile&quot;:&quot;1&quot;,&quot;classic_row_gap&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:35,&quot;sizes&quot;:[]},&quot;classic_row_gap_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;classic_row_gap_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"posts.classic\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<link rel=\"stylesheet\" href=\"https:\/\/mbioworks.com\/wp-content\/plugins\/elementor-pro\/assets\/css\/widget-posts.min.css\">\t\t<div class=\"elementor-posts-container elementor-posts elementor-posts--skin-classic elementor-grid\">\n\t\t\t\t<article class=\"elementor-post elementor-grid-item post-9922 post type-post status-publish format-standard has-post-thumbnail hentry category-wetlab tag-16s tag-18s tag-community-profiling tag-dna tag-sample tag-service tag-workflow\">\n\t\t\t\t<a class=\"elementor-post__thumbnail__link\" href=\"https:\/\/mbioworks.com\/?p=9922\" >\n\t\t\t<div class=\"elementor-post__thumbnail\"><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"200\" src=\"https:\/\/mbioworks.com\/wp-content\/uploads\/2026\/01\/primers2-300x200.png\" class=\"attachment-medium size-medium wp-image-9928\" alt=\"\" srcset=\"https:\/\/mbioworks.com\/wp-content\/uploads\/2026\/01\/primers2-300x200.png 300w, https:\/\/mbioworks.com\/wp-content\/uploads\/2026\/01\/primers2-768x512.png 768w, https:\/\/mbioworks.com\/wp-content\/uploads\/2026\/01\/primers2.png 800w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/div>\n\t\t<\/a>\n\t\t\t\t<div class=\"elementor-post__text\">\n\t\t\t\t<h3 class=\"elementor-post__title\">\n\t\t\t<a href=\"https:\/\/mbioworks.com\/?p=9922\" >\n\t\t\t\tPCR Primers for Microbial Community Profiling\t\t\t<\/a>\n\t\t<\/h3>\n\t\t\t\t<div class=\"elementor-post__meta-data\">\n\t\t\t\t\t<span class=\"elementor-post-date\">\n\t\t\tJanuary 14, 2026\t\t<\/span>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-post__excerpt\">\n\t\t\t<p>PCR primer choice shapes what microbial community profiling detects and how comparable results are across studies. Here is a quick reference list of commonly used primers for major targets\u201416S rRNA (bacteria\/archaea), ITS (fungi), and other standard marker genes.<\/p>\n\t\t<\/div>\n\t\t\t\t\t<div class=\"elementor-post__read-more-wrapper\">\n\t\t\n\t\t<a class=\"elementor-post__read-more\" href=\"https:\/\/mbioworks.com\/?p=9922\" >\n\t\t\tRead More \u00bb\t\t<\/a>\n\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/article>\n\t\t\t\t<article class=\"elementor-post elementor-grid-item post-8344 post type-post status-publish format-standard has-post-thumbnail hentry category-wetlab tag-equipment\">\n\t\t\t\t<a class=\"elementor-post__thumbnail__link\" href=\"https:\/\/mbioworks.com\/?p=8344\" >\n\t\t\t<div class=\"elementor-post__thumbnail\"><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"195\" src=\"https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/05\/autoclaver-s-300x195.jpg\" class=\"attachment-medium size-medium wp-image-8348\" alt=\"\" srcset=\"https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/05\/autoclaver-s-300x195.jpg 300w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/05\/autoclaver-s-1024x666.jpg 1024w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/05\/autoclaver-s-768x499.jpg 768w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/05\/autoclaver-s.jpg 1200w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/div>\n\t\t<\/a>\n\t\t\t\t<div class=\"elementor-post__text\">\n\t\t\t\t<h3 class=\"elementor-post__title\">\n\t\t\t<a href=\"https:\/\/mbioworks.com\/?p=8344\" >\n\t\t\t\tOperational Manual for Bentchtop Autoclaver\t\t\t<\/a>\n\t\t<\/h3>\n\t\t\t\t<div class=\"elementor-post__meta-data\">\n\t\t\t\t\t<span class=\"elementor-post-date\">\n\t\t\tMay 26, 2024\t\t<\/span>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-post__excerpt\">\n\t\t\t<p>A collection of operational manuals for essential instrument in microbiology\/microbial genomics laboratories.<\/p>\n\t\t<\/div>\n\t\t\t\t\t<div class=\"elementor-post__read-more-wrapper\">\n\t\t\n\t\t<a class=\"elementor-post__read-more\" href=\"https:\/\/mbioworks.com\/?p=8344\" >\n\t\t\tRead More \u00bb\t\t<\/a>\n\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/article>\n\t\t\t\t<article class=\"elementor-post elementor-grid-item post-8273 post type-post status-publish format-standard has-post-thumbnail hentry category-wetlab tag-dna tag-sample tag-service tag-workflow\">\n\t\t\t\t<a class=\"elementor-post__thumbnail__link\" href=\"https:\/\/mbioworks.com\/?p=8273\" >\n\t\t\t<div class=\"elementor-post__thumbnail\"><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"167\" src=\"https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/microbiome-mbioworks2-1-300x167.jpg\" class=\"attachment-medium size-medium wp-image-8338\" alt=\"\" srcset=\"https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/microbiome-mbioworks2-1-300x167.jpg 300w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/microbiome-mbioworks2-1-1024x569.jpg 1024w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/microbiome-mbioworks2-1-768x427.jpg 768w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/microbiome-mbioworks2-1-1536x853.jpg 1536w, https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/microbiome-mbioworks2-1.jpg 2047w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/div>\n\t\t<\/a>\n\t\t\t\t<div class=\"elementor-post__text\">\n\t\t\t\t<h3 class=\"elementor-post__title\">\n\t\t\t<a href=\"https:\/\/mbioworks.com\/?p=8273\" >\n\t\t\t\tDNA Extraction From Microbiomes\t\t\t<\/a>\n\t\t<\/h3>\n\t\t\t\t<div class=\"elementor-post__meta-data\">\n\t\t\t\t\t<span class=\"elementor-post-date\">\n\t\t\tApril 22, 2024\t\t<\/span>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-post__excerpt\">\n\t\t\t<p>High-quality DNA extraction is crucial for any microbiome sequencing project. At mBioWorks, we use reliable and cost-effective commercial microbiome DNA extraction kits to isolate pure DNA from all types of microbiome samples.<\/p>\n\t\t<\/div>\n\t\t\t\t\t<div class=\"elementor-post__read-more-wrapper\">\n\t\t\n\t\t<a class=\"elementor-post__read-more\" href=\"https:\/\/mbioworks.com\/?p=8273\" >\n\t\t\tRead More \u00bb\t\t<\/a>\n\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/article>\n\t\t\t\t<article class=\"elementor-post elementor-grid-item post-8126 post type-post status-publish format-standard has-post-thumbnail hentry category-wetlab tag-dna tag-sample tag-service tag-workflow\">\n\t\t\t\t<a class=\"elementor-post__thumbnail__link\" href=\"https:\/\/mbioworks.com\/?p=8126\" >\n\t\t\t<div class=\"elementor-post__thumbnail\"><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/mbioworks.com\/wp-content\/uploads\/2023\/04\/microbialworld-300x300.png\" class=\"attachment-medium size-medium wp-image-8124\" alt=\"\" \/><\/div>\n\t\t<\/a>\n\t\t\t\t<div class=\"elementor-post__text\">\n\t\t\t\t<h3 class=\"elementor-post__title\">\n\t\t\t<a href=\"https:\/\/mbioworks.com\/?p=8126\" >\n\t\t\t\tDNA Extraction From Microbial Pure Cultures\t\t\t<\/a>\n\t\t<\/h3>\n\t\t\t\t<div class=\"elementor-post__meta-data\">\n\t\t\t\t\t<span class=\"elementor-post-date\">\n\t\t\tApril 21, 2024\t\t<\/span>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-post__excerpt\">\n\t\t\t<p>High-quality DNA extraction is crucial for any microbial genome sequencing project. The mature industry of commercial DNA extraction kit offers a wide range of options. We utilize the most reputable and cost-effective DNA extraction kits, prioritizing both performance and affordability.<\/p>\n\t\t<\/div>\n\t\t\t\t\t<div class=\"elementor-post__read-more-wrapper\">\n\t\t\n\t\t<a class=\"elementor-post__read-more\" href=\"https:\/\/mbioworks.com\/?p=8126\" >\n\t\t\tRead More \u00bb\t\t<\/a>\n\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/article>\n\t\t\t\t<\/div>\n\n\n\t\t\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>We keep a maximum transparency and openness in what we do in the laboratory and on our bioinformatics servers. With these efforts, we wish to constantly improve our workflow and build trust in our customers.<\/p>\n","protected":false},"author":1,"featured_media":7552,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[16],"tags":[61],"class_list":["post-8092","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-protocols","tag-wetlab"],"_links":{"self":[{"href":"https:\/\/mbioworks.com\/index.php?rest_route=\/wp\/v2\/posts\/8092","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mbioworks.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/mbioworks.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/mbioworks.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/mbioworks.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=8092"}],"version-history":[{"count":34,"href":"https:\/\/mbioworks.com\/index.php?rest_route=\/wp\/v2\/posts\/8092\/revisions"}],"predecessor-version":[{"id":8748,"href":"https:\/\/mbioworks.com\/index.php?rest_route=\/wp\/v2\/posts\/8092\/revisions\/8748"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/mbioworks.com\/index.php?rest_route=\/wp\/v2\/media\/7552"}],"wp:attachment":[{"href":"https:\/\/mbioworks.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=8092"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/mbioworks.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=8092"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/mbioworks.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=8092"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}